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ENZECO® TRYPSIN/CHYMOTRYPSIN 1:1 is a beige to tan, amorphous powder that is derived from bovine pancrease. All glands are of North American origin. Both Trypsin, 3.4.21.4, and Chymotrypsin 3.4.21.1, are classed as serine Proteases.

Chymotrypsin preferentially cleaves Tyr, Tryp, Phe, Leu. The pH optimum is 8 - 9 with a temperature optimum of 35 C.

Typsin preferentially cleaves Arg and Lys. It's pH optimum is 8 - 9 with a temperature optimum of 45 C.

ACTIVITY

The activity of the enzyme is determined by the method published in the United States Pharmacopoeia.

The Typsin activity is approximately 1000 Units/milligram. A unit of activity is defined as the amount of enzyme causing an increase in absorbance at 253 nm of 0.003 per minute at 25 degrees Celsius

The Chymotrypsin activity is approximately 1000 Units/ milligram. A unit of activity is defined as the amount of enzyme causing a decrease in absorbance at 237 nm of 0.0075 per minute at 25 degrees Celsius.

STORAGE

Store in low humidity at less than 20 C.

Enzyme Development Corporation, 2 Penn Plaza , Suite 2439, New York, NY 10121-0034. Tel: 212-736-1580; Fax: 212-279-0056.