News | December 15, 2017

Limagrain Céréales Ingrédients (LCI) And Novolyze Partner To Strengthen Safety Of Flour Products

Limagrain Céréales Ingredients has recently partnered with Novolyze to reinforce the microbiological control of its proprietary heat process for flours: Farigel. In order to confirm the effectiveness of the Farigel process, Limagrain Céréales Ingrédients chose to use the innovative products developed by Novolyze. The methodology utilizes harmless bacteria that mimic the behavior of foodborne pathogens under different kinds of stress.

Jena Roberts, VP of Business Development at Novolyze stated that, “We are honored to partner with a prestigious company of Limagrain’s stature as they are the 4th largest seed company in the world. Limagrain is a also a unique partner for Novolyze as they are vertically integrated from seed to flour to cereal ingredients to bakery products. We service the same product categories so this is a very synergistic partnership.”

Even though foodborne pathogens have a small probability to grow in low moisture foods like flour, they can survive for many years due to the low water activity. Pathogen inactivation is usually obtained by the application of a preventive treatment. The Farigel process developed by Limagrain Céréales Ingrédients applies a thermal stress on flours and is therefore considered a preventive control for foodborne pathogens like Salmonella or Escherichia coli.

Roberts added, “the surrogate bacteria developed by Novolyze have a slightly higher resistance compared with the target pathogens to kill. Our surrogates, because they are not pathogenic, can be used directly in-plant or at a pilot scale to conduct tests. The introduction of a pathogen in an industrial environment is strictly forbidden. That is why our clients decide to use our products”.

Thierry Desgrousilliers, Quality Manager at LCI added that “the Farigel process is the result of several years of internal development and optimization. Consequently, it is very difficult to reproduce at a laboratory or pilot scale in order to carry out microbial tests. That is the reason why we decided to use Novolyze’s surrogate organisms. The use of Novolyze’s products allowed us to test the efficacy of our process directly in its specific industrial environment, make validation work extremely straightforward and simple”.

A validation project with surrogate microorganisms commonly includes three major steps. The first consists in the definition of a validation strategy. After that, the resistance of the surrogate is verified at lab scale. Roberts explained that “this step is particularly important and determines the whole project validity. As a matter of fact, Codex Alimentarius 2008 clearly underlines this point. In the case of the Farigel process, Novolyze performed an experimental study with Limagrain’s flour. This test enabled to verify that the selected surrogate had a similar resistance to foodborne pathogens. The 3rd and last step consisted in performing validation trials directly on the Farigel process at factory scale. The tested flour was inoculated with the Novolyze surrogate and then distributed in the Farigel process. Post-process samples were collected and analyzed, which enabled to calculate the capacity of the Farigel process to inactivate pathogens.”

Roberts added that “the main advantage of our surrogates is that they are dried and ready to use. Their inoculation with flour is obtained by blending. Our bacteria don’t require a laboratory to be used. It enables to inoculate very easily and precisely small as well as big quantities of food, most of the time directly at the process facilities”.

The results enabled LCI to confirm that the Farigel process was capable of eliminating 5-log of Salmonella. The Novolyze dry surrogate is uniquely used in the production equipment to create “gold-standard” data for the process validation which is a pivotal benefit for regulatory inspections. This study reinforced the control of the Farigel process. Desgrousilliers commented: “this is the first time we objectively confirmed that our process is capable of inactivating foodborne pathogens. Beyond the interests linked to the texturization properties of Limagrain’s flours, this confirms the effectiveness of our process as a microbiological critical control point. We give ourselves a little more time before launching new tests with the same method.”

Surrogate organisms are a key tool to ensure food safety. They are used for regulatory compliance (FSMA etc.). However, they can also be of strong help for product and process innovation. Roberts concluded: “the use of our surrogates enables to obtain a more precise vision of a process effectiveness. In a significant number of cases, the use of our products also enabled to optimize treatment parameters with the following outcomes: improved organoleptic and nutritional quality of food, reduced energy expenses and productivity gains.”

Both companies are excited that a validation project can also improve efficiency via process optimization. Please follow us on LinkedIn for updates on this important partnership.

About Limagrain Céréales Ingrédients (LCI)
Limagrain is a cooperative group specialized in seeds and cereal products. Limagrain is the 4th largest seed company in the world and the 2nd largest French baker. The company’s revenues reached 2.6 billion Euros in 2016. Limagrain hires more than 10,000 employees including more than 2,000 employees in over 56 countries.

Limagrain Céréales Ingrédients (LCI) is one of the 13 different Business Units of Limagrain group. As a creator of functional cereal ingredients for the agri-food industry, LCI have a turnover of more than €100M, of which 30% from export market. LCI transform every year more than 200,000 tonnes of cereals for the different food markets: bakery, snacks, breakfast cereals, ready meals, dairy products and nutrition. The company has more than 250 employees and 6 production sites.

About Novolyze
Novolyze is a food safety company that offers solutions to help the food industry manufacture safer food, while ensuring strong compliance with international Food Safety & Quality standards such as F.D.A. Food Safety Modernization Act. The Novolyze portfolio of surrogates and services evaluates the inactivation of foodborne pathogens under different kinds of stress (heat etc.). Novolyze has developed a cutting-edge portfolio of dry non-pathogenic surrogate bacteria that mimic the behavior of foodborne pathogens.

Recently, Novolyze was chosen to participate in TERRA, the Food + Ag Tech Accelerator as one of 18 food and agriculture startups that are deemed most disruptive and progressive. Terra is a collaboration of Rabobank, Rocketspace, Nestle, AgroFresh, BASF, Beta San Miguel, Givaudan, GrainCorp, Griffith Foods, Grupo Azucarero Mexico/CULTIBA, and OSI Group. Additionally, Novolyze was awarded funds from the French Government to develop validation innovations for Pre-Cut, Fresh and Frozen Fruits and Vegetables. This summer, Novolyze received the Food Safety Innovation Award from the International Association for Food Protection (IAFP) and was ranked in the top 9% of start-up companies by EarlyMetrics. For more information, visit

SOURCE: Novolyze